Holzhauer is best known for being a 32-time champion on Jeopardy! in 2019, where he accumulated $2,464,216. He currently holds the top 16 records for most cash won in a single episode on that show. And twice, he broke the single-day cash record; the first time, he won $110,914, and the second time, he broke his own record with $131,127.
Holzhauer also competed in the Jeopardy! Greatest of All Time Tournament and became a runner-up by inputing a wrong answer at the final match causing Ken Jennings to win the Tournament. He also competed in the Jeopardy! Masters Tournament and won in the first season and came in 3rd place in the 2nd season, causing to let Victoria Groce win. He competed in the Go Fact Your podcast in 2022 with Victoria Groce and won by being only one point ahead of her.